rescue plasmids from single COS-7 cells

Matthias Liniger matthias.liniger at
Thu Oct 7 09:35:47 EST 1999

I transfected COS-7 cells with a plasmid library.
I tell you: It's not trivial to scrape away single positive
(immunoscreened) cells, isolate plasmids
and electroporate into E. coli.

Any Ideas?

More information about the Methods mailing list