Marker in bacterial chromosome or plasmid?

Edgar Valencia edgar at CIFN.UNAM.MX
Thu Oct 7 12:47:24 EST 1999


Hi Juamber

Locating transposon insertion in plasmid 
SNIP

> I don't have facilities for pulsed field electrophoresis, nor for CsCl.
> I have tried to "cure" the transformed strain using high incubation
> temperatures without success.
> Do you have any idea on how to check out the location of this
> integration? 

Uhmm what about doing and Eckhardt type gel and southern blot. In this gel
you'll see just the plasmid (the chromosome will be broken and will
appear just like a "smear" in the bottom part of the gel). We use this
technique for Rhizobium's plasmid analysis and it works pretty well.


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