Isopropanol vs EtOH
gwmartin at fas.harvard.edu
Thu Oct 7 14:51:43 EST 1999
rogier <stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid> wrote:
> simple. you only need 1 vol of isoprop, instead of 2 vol. EtOH. this
> can be the difference between spinning in eppies or looking for exotic
> big tubes and spinners to fit them in. or dividing your DNA among
> eppies so you have twice as many samples to fight with.
> and yeah, isoprop precipitation gives cleaner DNA cos you don't need to
> add tons of salt.
> and its faster, too.
But harder to get rid of after precipitation than ethanol. I think that's
why at least one ethanol wash is suggested after isoprop precipitation.
More information about the Methods