monoclonal antibodies

Scott Minns smeg2cletus at my-deja.com
Thu Oct 7 16:27:31 EST 1999


You could start praying to god of choice?? You may have to grow up the
last vial and then subculture to find Mab producing cells. Are you sure
there is no low grade myco infection?? This can stop hybridomas from
producing but they also appear to be sick.

Best of luck,

scott m
>
> Hi everyone,
>
> I am currently culturing hybridomas for antibody production.  The only
> problem is, the cells appear to have stopped producing the antibody.
This
> is my last vial of cells so I NEED them to produce.  Does anybody know
> what I can do to "switch" the antibody production back on ??  I am
using
> DMEM-HT with 20% FCS as the culture media.
>
> Thanks in advance.
>
> Tom
>
> -----------------------------------------------
> Tom La, PhD Student
>
> Division of Veterinary and Biomedical Sciences
> Murdoch University
> Murdoch, Western Australia, 6150
>
> Tel :  61 08 9360 2247
> Facsimile :  61 08 93104144
> Email :  tom_la at central.murdoch.edu.au
>
> And the end of all our exploring
> Will be to arrive where we started
> And know the place for the first time.
> -- T.S. Elliot --
> -----------------------------------------------
>
>


Sent via Deja.com http://www.deja.com/
Before you buy.



More information about the Methods mailing list