His-tag and Western blot

Ranga Uday Kumar uday at JNCASR.AC.IN
Fri Oct 8 04:40:54 EST 1999


    Could any body give me a protocol to detect protein expression in E.
coli
using Histidine tag by western blot? Can His-tag be used for western
blot?
We are in a situation that only His-tag is available for the detection
of
our protein. Thank you.




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