His-tag and Western blot
Dr Dave Parcej
parcej at biophys.mpg.de
Fri Oct 8 06:39:09 EST 1999
Sigma sell an anti-polyhis monoclonal (clone His-1) which works very well
for us in Westerns.
In article <37FDBA6B.318739B3 at jncasr.ac.in>, uday at JNCASR.AC.IN (Ranga Uday
> Could any body give me a protocol to detect protein expression in E.
> using Histidine tag by western blot? Can His-tag be used for western
> We are in a situation that only His-tag is available for the detection
> our protein. Thank you.
More information about the Methods