His-tag and Western blot

Dr Dave Parcej parcej at biophys.mpg.de
Fri Oct 8 06:39:09 EST 1999


Hi
Sigma sell an anti-polyhis monoclonal (clone His-1) which works very well
for us in Westerns.
Dave

In article <37FDBA6B.318739B3 at jncasr.ac.in>, uday at JNCASR.AC.IN (Ranga Uday
Kumar) wrote:

>     Could any body give me a protocol to detect protein expression in E.
> coli
> using Histidine tag by western blot? Can His-tag be used for western
> blot?
> We are in a situation that only His-tag is available for the detection
> of
> our protein. Thank you.



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