His-tag and Western blot

[Eric Chace Olivares]

Neil McKenna (nmckena at ottawa.com) wrote:
: I use Quiagen's anti-tetra-his antibody to detect HisX6 in Western
: blotting. I haven't noticed any problem in detecting. (I have no
: affiliation with them, etc).

Anyone using Clontechs 6HIS-mAbs that are pre conjugated to AP or HRP?
Seems like a simple way to go right to detection.

-=Eric

-- 
Eric C. Olivares
Department of Genetics
Stanford University School of Medicine
olivares at stanford.edu
http://www.stanford.edu/~olivares/