His-tag and Western blot

Eric Chace Olivares olivares at leland.Stanford.EDU
Fri Oct 8 09:31:15 EST 1999

Neil McKenna (nmckena at ottawa.com) wrote:
: I use Quiagen's anti-tetra-his antibody to detect HisX6 in Western
: blotting. I haven't noticed any problem in detecting. (I have no
: affiliation with them, etc).

Anyone using Clontechs 6HIS-mAbs that are pre conjugated to AP or HRP?
Seems like a simple way to go right to detection.


Eric C. Olivares
Department of Genetics
Stanford University School of Medicine
olivares at stanford.edu

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