mouse from man on the PCR level

Mr Y. Schwartz yschwart at hgmp.mrc.ac.uk
Fri Oct 8 10:07:03 EST 1999


You may just use the STRs (Short Tandem Repeat, which are common in
forensic applications.
You may find the particular primer sequences in the net, just using any
net serching
server and the key word STR.
Good luck, Yuri.

On Thu, 7 Oct 1999, L. Scot Bastian Ph.D. wrote:

> Greetings all,
> 
>      We're going to do hematopoietic reconstitution studies using human
> cells in irradiated mice. The studies are a little too complex to fully
> describe here. This might seem to be an obvious question, but what is a
> good DNA marker to use detect human cells in the blood of mice using
> PCR.  The cells are likely to be scarce, so repetitive DNA that is
> present in abundance in human cells, and absent in mouse would be
> great.  If you can suggest specific primer pairs or specific references
> where this has been done I would be most grateful.
> 
> Thanks,
> L. Scot Bastian Ph.D.
> 
> 
> 




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