His-tag and Western blot

Chen Linan chenlinan at 263.NET
Fri Oct 8 11:20:42 EST 1999


At 02:40 99-10-8 -0700, you wrote:
>    Could any body give me a protocol to detect protein expression in E.
>coli
>using Histidine tag by western blot? Can His-tag be used for western
>blot?
>We are in a situation that only His-tag is available for the detection
>of
>our protein. Thank you.
>

I don't think anti-poly-his antibody could not be used in western blot
successfully. What I concern is 6-his mab from clonetec if can be used for
immunohistochemistry study. Any suggestion?

Chen Linan
Dept. Anatomy
Beijing Medical University




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