His TAG protein purification

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Mon Oct 11 07:11:57 EST 1999


>Who ever said Ni columns were a one step
>purification has a strange sence of humor

try 2 tags, GST and His.
after gluthatione and Ni-NTA (or the other way 'round) my proteins were
always clean.
Good luck,
Rogier


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