help about PCR failure 2

Jin-seon Im jim at U.ARIZONA.EDU
Mon Oct 11 19:59:04 EST 1999

hello, guys.

I looked at the template again, found out that template might form
secondary structure in the region where I designed primer for. Since I
have to use that specific region for cloning purpuse, I gotta figure this
out. Any suggestions wil be appreciated !!!!


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