Why DTT in RNA prep?

Peter pxpst2 at vms.cis.pitt.edu
Tue Oct 12 08:26:12 EST 1999


In article <3802C251.5DA7212C at musica.mcgill.ca>, Mano
<mdep at musica.mcgill.ca> wrote:

> Also note that, if you are using your RNA in the presence of RNAGuard or
> other RNase inhibitor, DTT is necessary for the inhibitor to function.  In
> the absence of DTT, the use of an RNase inhibitor is presumably useless.  I
> read this once in Methods in Enzymology (cannot recall the volume#)

Someone else said this as well earlier.  I thought it sounded peculiar but
do not doubt its truth.  Do you have any idea why it is needed?  It just
seems counter intuitive that a reducing agent is needed for the inhibitor
to function.

Regards,
Peter Pediaditakis
University of Pittsburgh
Dept. of Pathology



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