Isoleucine in HP 1000A peptide sequencer

Martin Jakt mjakt at
Wed Oct 13 04:59:35 EST 1999

Hi there,

I have sequenced a 19 amino acid tryptic peptide isolated from a brain
protein.   The sequence is really clean, indicating only one peptide
present, however at two cycles Ile is the highest peak but Phe is about
half the peak height.  Is this a question of the sequencing chemistry
upon hitting Ile or is this an allelic protein sequence?


ps. please email answers to erikmiljan at as I may not be able to
check this newsgroup. (this written from a friend's computer)

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