EGTA

Enrique Castro ecastro at eucmax.sim.ucm.es
Wed Oct 13 07:11:08 EST 1999



"Chong Wai Yin( Zhang Weixian)" wrote:
> 
> Hi people! Can anyone tell me are there any difference between using EDTA
> and EGTA in lysis buffer. Is the function of EGTA the same as EDTA? Thank
> you for answering.

EDTA binds both Ca2+ and Mg2+. EGTA is a chelator more or less selective
for Ca2+, it will not sequester Mg2+ from your solution.


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Enrique Castro
Dept. Bioquimica, 
Fac. Veterinaria, UCM.
Ciudad Universitaria. 28040 Madrid. Spain
Tel: 34-91-394 38 90
Fax: 34-91-394 39 09
e-mail: ecastro at eucmax.sim.ucm.es
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