PCR anomaly

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Oct 13 10:40:51 EST 1999

In article <38048B10.5286F948 at laplace.csb.yale.edu>,
  Mark Edward Bowen <mb at laplace.csb.yale.edu> wrote:
> <!doctype html public "-//w3c//dtd html 4.0 transitional//en">
> <html>
> Replacing most components is not difficult or all that costly, but surely
> one doesn't have to destroy the primers.&nbsp; There must be a way to "reclaim"
> them.&nbsp; Either phenol/CHCl3/EtOH or adding RNase should be able to
> clean them of any contaminant.&nbsp; Does anyone have a protocol for rescuing
> suspect primers?

Surely these days primers are cheap enough that is makes more sense to
make new ones than to try to reclaim suspect ones. (Heck, some people
make new ones when they just can't find them in the freezer!)


| Nick Theodorakis                              |
| nicholas_theodorakis at urmc.rochester.edu       |
| (previously theodorn at gusun.georgetown.edu)    |

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