Kinase in Ligation?

Karl Schmid kschmid at
Fri Oct 15 10:32:07 EST 1999

Hi everybody!

Does T4 kinase disturb my ligation? (e.g. by binding free 5prime ends) Is
it enough to heat inactivate or is a phenol/chloroform extraction needed?

HOw do you treat your PCR fragmants before cloning (blunt into pZero, a
vector that should not be dephosphorylated)

Thanks for your advice,


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