Kinase in Ligation?

Karl Schmid kschmid at zi.biologie.uni-muenchen.de
Fri Oct 15 10:32:07 EST 1999


Hi everybody!

Does T4 kinase disturb my ligation? (e.g. by binding free 5prime ends) Is
it enough to heat inactivate or is a phenol/chloroform extraction needed?

HOw do you treat your PCR fragmants before cloning (blunt into pZero, a
vector that should not be dephosphorylated)

Thanks for your advice,

Gregor



More information about the Methods mailing list