Gu-HCl/Acetic acid and PAGE

Peter pxpst2 at vms.cis.pitt.edu
Fri Oct 15 14:47:29 EST 1999


In article <3807722A.747985F9 at ottawa.com>, Neil McKenna
<nmckena at ottawa.com> wrote:

> Does anyone have any experience running samples that have been dissolved
> in 6M Gu-HCl/0.2 M acetic acid on SDS-PAGE gels? Will it dissolve the
> gel? Do the samples need to be precipitated?

bases and only bases will cause a acrylamide gel to depolymerize.

How big is the sample volume?
If it is small and you are running a discontinuous gel then I would
suspect that it will not affect to a large extent , the gel seperartion.

regards,
Peter Pediaditakis



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