cloning problem!

R. Jayakumar jakku at mrna.tn.nic.in
Sat Oct 16 01:32:10 EST 1999


try lambda dash II vector

jakku
----- Original Message ----- 
From: zarir <zarir at purdue.edu>
To: <methods at hgmp.mrc.ac.uk>
Sent: Thursday, October 14, 1999 2:16 AM
Subject: cloning problem!


> Hi all,
>          I am having a big headache cloning  a 7.2 kb long pcr fragment
> directionally (ecorI-bamHI) into a pCambia vector (for plant
> expression). I have tried a lot of things. Please pass me some
> suggestions from those who have cloned large framents. Please email me
> at zarir at purdue.edu
> zarir
> 

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