Gu-HCl/Acetic acid and PAGE

Peter pxpst2 at vms.cis.pitt.edu
Sat Oct 16 11:40:13 EST 1999


In article <l03010d00b42d4693e7ef@[200.12.133.36]>,
seanpat at FMED2.UNCU.EDU.AR (Sean Patterson) wrote:

>         A little lateral to the subject, but I seem to recall a protocol
> for extracting proteins from polyacrylamide gel fragments using 30% H2O2.
> Does anyone know if this does actually depolymerize a gel?

I think at high pH

OH- + H2O2 <---> H2O + HO2-

I would venture to guess that hydroperoxide ion would be pretty good at
depolymerizimg acrylamide.


Peter Pediaditakis



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