Tris buffer and the Biorad model 491 Preparative Electrophoresis
Dr P.P.E. Freestone
ppef1 at leicester.ac.uk
Mon Oct 18 05:56:19 EST 1999
We are using a Biorad model 491 Preparative Electrophoresis cell to
purify collagen. To optimise our purification, we'd like to collect
discrete peaks by connecting the cell tube outlet to a flow cell.
However, the problem is that the tris electrophoresis buffer that the
491 cell uses has a similar absorbance maximum to our protein (around
340 nm - collagen has very little UV absorbance at 280 nm). So, does
anyone out there know if other (non-tris) buffer systems exist which
will still give separations around pH 7-8, and which don't absorb so
strongly at 340 nm ?
Thanks everyone !
University of Leicester
ppef1 at le.ac.uk
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