Why DTT in in situ hybridization???

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Mon Oct 18 09:00:53 EST 1999


Hi,
trial and error taught that RNasin needs DTT to work, even tho the mech
is not clear. without RNasin, RNases may trim your probes, which may
then bind to undesired sequences. keep in mind that 58 C is not a very
high hybridization temperature, since RNA-RNA and RNA-DNA hybrids are
more stable than DNA-DNA.
Good luck,
Rogier
rogier at biogate.com


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