Bomerang Northerns - bands keep coming back!

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Mon Oct 18 09:28:58 EST 1999


this may solve your problem:
when you reprobe, add excess unlabeled previous probe/probes.
If excess unlabeled probe doesn't erase the bands, your washing was not
stringent enough.
if your probe doesn't wash off properly, you can attack it with
(RNase-free!) DNase before reprobing. Don't forget to heat-inactivate
the DNase before adding your new probe...
good luck,
Rogier
rogier at biogate.com


* Sent from RemarQ http://www.remarq.com The Internet's Discussion Network *
The fastest and easiest way to search and participate in Usenet - Free!




More information about the Methods mailing list