Heat stability of RNA

John Scott Meschke Scott_Meschke at unc.edu
Mon Oct 18 13:13:22 EST 1999


List-
        This may seem like a silly question.  What is the probability
that RNA be damaged by exposure to 95C for 5 min?  I realize that for
heat release RT-PCR of viral RNA this is routine to open the viral
capsid.  However, when doing an RNA extraction prior to RT my gut
reaction is to still heat the RNA before addition of the reverse
transcriptase in order to reduce secondary structure.  Is this heating
potentially problematic?

Curious,

Scott
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