Why DTT in in situ hybridization???
barna at koki.hu
Tue Oct 19 09:32:14 EST 1999
Maybe you are absolute right, but in situ hybridization solutions do not
contain RNAsin :)))
and the probe
>trial and error taught that RNasin needs DTT to work, even tho the mech
>is not clear. without RNasin, RNases may trim your probes, which may
>then bind to undesired sequences. keep in mind that 58 C is not a very
>high hybridization temperature, since RNA-RNA and RNA-DNA hybrids are
>more stable than DNA-DNA.
>rogier at biogate.com
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