Why DTT in in situ hybridization???

Istvan Barna barna at koki.hu
Tue Oct 19 09:32:14 EST 1999


Maybe you are absolute right, but in situ hybridization solutions do not
contain RNAsin :)))

it contains:

Formamide
Dextran
Nacl
Denhard's
TRIS
EDTA
tRNS
DTT
and the probe

Thanks,

Istvan Barna


>Hi,
>trial and error taught that RNasin needs DTT to work, even tho the mech
>is not clear. without RNasin, RNases may trim your probes, which may
>then bind to undesired sequences. keep in mind that 58 C is not a very
>high hybridization temperature, since RNA-RNA and RNA-DNA hybrids are
>more stable than DNA-DNA.
>Good luck,
>Rogier
>rogier at biogate.com



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