synthetic gene

William Sun william at neuro.usc.edu
Tue Oct 19 15:53:46 EST 1999


Hello all,

Does anyone have experience assembling a cDNA from a set of overlapping
oligonucleotides?  How long can I make the primer and how big an overlap?
Can I anneal all the primers and vector together in one step?  Any advice
or reference would be greatly appreciated.  

William 

-- 
William Sun, Ph.D               
Metabasis Therapeutics, Inc.           Phone: (619) 622-3930
9390 Towne Centre Drive                FAX:   (619) 622-5583
San Diego, CA 92121                    Email: william at alum.mit.edu 



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