synthetic gene

William Sun william at
Tue Oct 19 15:53:46 EST 1999

Hello all,

Does anyone have experience assembling a cDNA from a set of overlapping
oligonucleotides?  How long can I make the primer and how big an overlap?
Can I anneal all the primers and vector together in one step?  Any advice
or reference would be greatly appreciated.  


William Sun, Ph.D               
Metabasis Therapeutics, Inc.           Phone: (619) 622-3930
9390 Towne Centre Drive                FAX:   (619) 622-5583
San Diego, CA 92121                    Email: william at 

More information about the Methods mailing list