william at neuro.usc.edu
Tue Oct 19 15:53:46 EST 1999
Does anyone have experience assembling a cDNA from a set of overlapping
oligonucleotides? How long can I make the primer and how big an overlap?
Can I anneal all the primers and vector together in one step? Any advice
or reference would be greatly appreciated.
William Sun, Ph.D
Metabasis Therapeutics, Inc. Phone: (619) 622-3930
9390 Towne Centre Drive FAX: (619) 622-5583
San Diego, CA 92121 Email: william at alum.mit.edu
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