GSH measurement

John Thompson jrt at home.com
Tue Oct 19 17:51:29 EST 1999


"Dr. Holger Olbrück" <holger.olbrueck at ruhr-uni-bochum.de> wrote:

>Hello,
>who knows a good method for the determination of intracellular GSH?
>Thanks
>Holger
>

DTNB will certainly work but is not specific.  It will react with DTT
or mercaptoethanol, if present, and maybe free Cysteines in your
proteins.  

I'd try to find an enzymatic method that should be more specific.  Not
sure how but bet it's been done.

Yours,
John Thompson



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