krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Oct 20 11:47:56 EST 1999
In bionet.molbio.methds-reagnts Pedro Silva <Pedro.Silva at nospamepr.bc.wau.nl> wrote:
> I have partially purified a membrane protein from a hyperthermophilic
> organism. The protein has been solubilized with 0.4% DOC, then
> precipitated w/ 15% ammonium sulfate and resuspended in 0.4% DOC
> -containing buffer.
> Then I tried to run a SDS-Phast Gel with it, but the protein did not
> migrate at all. I had boiled it for 10 min w/ SDS and
Membrane proteins often have the nasty property of forming large
insoluble aggregates when being boiled in the presence of SDS. Try
incubating it in 5x Laemmli at approx. 30°C for a while.
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: phak004 at rzbox.uni-wuerzburg.de SP4 */
/* "Science is the game we play with God to find out what His rules are." */
More information about the Methods