Nonspecific DNA binding to Dynabeads?
chris at hobbes.chem.ualberta.ca
Thu Oct 21 12:54:16 EST 1999
I've just begun using Dynabeads for PCR applications, and have been
having some problems with what appears to be non-specific binding of
non-biotinylated DNA fragments to the beads, even after repeated washes
of large volumes in 2 M NaCl. The application that I am developing
requires virtually absolute specificity - that is, anything that isn't
biotinylated OUGHT NOT TO BE THERE. Has anyone experienced similar
problems, or have any suggestions/words of wisdom/references???
Please reply to chris at hobbes.chem.ualberta.ca
Thanks, in advance!
University of Alberta
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