Nonspecific DNA binding to Dynabeads?

Chris Dambrowitz chris at hobbes.chem.ualberta.ca
Thu Oct 21 12:54:16 EST 1999


I've just begun using Dynabeads for PCR applications, and have been
having some problems with what appears to be non-specific binding of
non-biotinylated DNA fragments to the beads, even after repeated washes
of large volumes in 2 M NaCl.  The application that I am developing
requires virtually absolute specificity - that is, anything that isn't
biotinylated OUGHT NOT TO BE THERE.  Has anyone experienced similar
problems, or have any suggestions/words of wisdom/references???  

Please reply to chris at hobbes.chem.ualberta.ca

Thanks, in advance!

Chris Dambrowitz
University of Alberta



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