enterokinase

Dr Dave Parcej parcej at biophys.mpg.de
Fri Oct 22 06:17:37 EST 1999


Alexandre,
We have used EKmax from Invitrogen with great success.
cheers
Dave

In article <380F3EBB.FB27A997 at biocfebs.unizh.ch>,
amooser at BIOCFEBS.UNIZH.CH (Alexandre) wrote:

> hi everybody,
> 
> I want to express a protein linked to a very soluble fusion partner. To
> remove the fusion partner at the end of the purification, I want to
> engineer a bovine enterokinase site, which cleaves (specifically ?!?) a
> given sequence.
> Does anybody have experience with that protease ? which one (from which
> supplier) performs best ? do you have any idea about the conditions
> (ions, pH...) I have to use to get an efficient cleavage.
> 
> thanx for any help
> 
> Alexandre Mooser
> Department of Biochemistry
> University of Zurich
> Switzerland



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