Why DTT in in situ hybridization???

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Fri Oct 22 08:21:18 EST 1999


>But I use DIG-labelled riboprobes!! Do you think there is any
protective effect of DTT on this system, too


no. too much DTT may even kill the cysteine links in the DIG antibody

Rogier


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