Mechanical shearing of DNA

Helen Dickens hfd at mrc-lmb.cam.ac.uk
Fri Oct 22 08:39:29 EST 1999


Hi,
We are making  a library with inserts in the region of 15 - 25 kb. Does
anyone have any techniques for reproducibly obtaining sheared genomic
DNA in this sort of range? I have tried a few basic ones but am worried
about being consistent between batches.  As this is a one-off (we hope!)
effort, we'd rather not buy any specialised equipment if possible. We
think we should be able to get what we want with needles and syringes or
possibly nebulisers?
Thanks for your help,
H. Dickens




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