TAQ/PWO ratio in LA PCR

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Oct 22 09:35:38 EST 1999

In article <7uplun$jqh$1 at news.rrz.Uni-Koeln.DE>, mkroiher at biolan.uni-
koeln.de writes
>Who has experience with the ratio of TAQ/PWO in LA PCR like in the
>Expand PCR system from Roche (Boehringer)? Is it about the same as 
>for KlenTAQ/PFU reported from Barnes. 

The DNA sequence in Genbank for Pwo is the same as the sequence in
Genbank for Pfu, so one would guess that the ratio is the same as that
claimed in Wayne Barnes's US and European patents now owned by Takara

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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