Full double digestion?

Chen Linan chenlinan at 263.NET
Sat Oct 23 05:11:25 EST 1999


Hi,

I am digesting plasmid with two enzymes and recombinate a DNA fragment with
the digested plasmid, but am troubled with self-circulated plasmid due to
partial digestion so that can not select recombinant colony. Is there any
one tell me how to make sure that I have got fully double digested plasmid?
Thanks,

Chen linan
Dept. Anatomy
Beijing Medical University





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