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subcellular fractionation of insect cells

Merike Meier meerike at KBFI.EE
Mon Oct 25 09:38:49 EST 1999


Hello - Does anybody can help with working protocol about differential or 
gradient centrifugation for subcellular fractionation of insect cells 
(Sf9 cell line)? Also, any information about the densities or/and 
sedimentation coefficients of different types of INSECT cell organelles 
would be helpful.
Unfortunately, I have no possibility for electron microscopy and no 
knowledge about marker enzymes for insect cell organelles to design a 
good experimental protocol for determining the insect cell subcellular 
fractions.

Merike Meier, PhD student
meerike at kbfi.ee



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