subcellular fractionation of insect cells

[Merike Meier]

Hello - Does anybody can help with working protocol about differential or 
gradient centrifugation for subcellular fractionation of insect cells 
(Sf9 cell line)? Also, any information about the densities or/and 
sedimentation coefficients of different types of INSECT cell organelles 
would be helpful.
Unfortunately, I have no possibility for electron microscopy and no 
knowledge about marker enzymes for insect cell organelles to design a 
good experimental protocol for determining the insect cell subcellular 
fractions.

Merike Meier, PhD student
meerike at kbfi.ee