In vitro transcription and crud extract from E.coli?

Mike Marr mtm5 at cornell.edu
Mon Oct 25 11:03:44 EST 1999


These are two good references for using Crude extract from E. coli for
transcription. 


Li J, et al.          
       NusG, a new Escherichia coli elongation factor involved in
transcriptional antitermination by the N protein of phage
       lambda. 
       J Biol Chem. 1992 Mar 25;267(9):6012-9.

     Goda Y, et al.           
       Efficient modification of E. coli RNA polymerase in vitro by the
N gene transcription antitermination protein of
       bacteriophage lambda. 
       Nucleic Acids Res. 1985 Apr 11;13(7):2569-82.

Mike Marr 
      

Yoram Gerchman wrote:
> 
> Greeting Netters
> I'm looking for a method for making a crude extract from E.coli. The idea
> is to look for missing components that affect the transcription of our
> gene.
> Thanks Yoram
> .........................................................................
> "Support bacteria - they're the only culture some people have"
> 
>                                      (anonymous)
> 
> Yoram Gerchman
> Division of Microbial & Molecular Ecology
> The Institute of Life Sciences
> Hebrew University of Jerusalem
> Givat Ram, Jerusalem 91904, Israel



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