Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Tue Oct 26 01:02:31 EST 1999

"Frank O. Fackelmayer" wrote:

> Bart Frank wrote:
> > What does "NTCB" stand for? It is a cys-specific protease.
> > Thanks
> > Bart
> Hi Bart,
> NTCB stands for 5-thiocyanobenzoic acid. It is a reagent that cyanylates
> cysteine residues in proteins and make the peptide bond N-terminally
> from the modified cysteine susceptible to cleavage at slightly alkaline
> conditions (pH 9-10). Check Medline for references. A starting point may
> be Daniel et al. (1997), JBC 272(43):26934-23939
> Frank

The N in NTCB is "nitro", so the full name of NTCB is
2-nitro-5-thiocyanobenzoic acid. Sorry.

More information about the Methods mailing list