NTCB??

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Tue Oct 26 01:02:31 EST 1999



"Frank O. Fackelmayer" wrote:

> Bart Frank wrote:
>
> > What does "NTCB" stand for? It is a cys-specific protease.
> > Thanks
> > Bart
>
> Hi Bart,
> NTCB stands for 5-thiocyanobenzoic acid. It is a reagent that cyanylates
> cysteine residues in proteins and make the peptide bond N-terminally
> from the modified cysteine susceptible to cleavage at slightly alkaline
> conditions (pH 9-10). Check Medline for references. A starting point may
> be Daniel et al. (1997), JBC 272(43):26934-23939
>
> Frank

OOPS!
The N in NTCB is "nitro", so the full name of NTCB is
2-nitro-5-thiocyanobenzoic acid. Sorry.
Frank




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