ATA and RT-PCR
ckafer at iastate.edu
Wed Oct 27 20:58:02 EST 1999
I have a great protocol for RNA extraction from plant tissue that
utilizes Aurintricarboxylic Acid (ATA) as an RNAse inhibitor
(Biochemistry 19; 4299). ATA inhibits the formation of
protein-nucleic acid complexes...at least with RNAse.
So my question is, is this a general inhibition of most nucleic
acid-protein complexes or just RNAse? Specifically, will this kill
Any insights will be appreciated!
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