Q:Alternative to RNAse protection

ttu00k at sp2.power.uni-essen.de ttu00k at sp2.power.uni-essen.de
Fri Oct 29 04:52:23 EST 1999


We have determined three different transcription start sites for a gene
and now want to quantify the relative abundance of transcripts from each
of the starting points. Since we wanted to avoid, if possible,
establishing quantitative RT-PCR or RNAse protection is anybody aware of
a "simple" technique to answer this question?
Many thanks in advance.

Alexander Schramm
Universitätsklinikum Essen
Innere Klinik/Tumorforschung

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