odd observation about DNA

Andrew Leung leungkc at glink.net.hk
Fri Oct 29 09:09:29 EST 1999


band prep is done using silica glassmilk from BIO101 Gene Clean Kit.
Other procedure is standard.


Gerd  ¼¶¼g©ó¤å³¹ <7vbi0i$eh6$1 at news.uit.no>...
>Need to know a little more about what you have been doing.
>What have you done to the DNA between 1. and 2. gel? And what methods have
>been used?
>
>any posibility for sticky ends finding each other?
>
>Gerd
>
>In article <7vao1a$5l9$1 at unix2.glink.net.hk>, "Andrew Leung"
><leungkc at glink.net.hk> wrote:
>
>
>>Hi all,
>>
>>I have band-prep a 1.1kb pcr product from agarose gel. Then I run the prep
>>in the agaorse gel again. But what I observed is a multiple bands of 1.1kb
>>bands. So...........how to explain this.
>>
>>The pcr product is 70% GC rich. Is it due to............imperfect
alignment
>>across individual amplicon.
>>
>>Andrew
>>
>>





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