Protein free blocking agent

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Oct 29 09:00:02 EST 1999


In article <38195DF5.8A326B2 at hotmail.com>, Fred <dynamin at hotmail.com>
wrote:
> We use PVP-40 (very cheap from Sigma) to block NC or PVDF for
> western
> blots.  Works extremely well.  Never tried it for hybridisations,
> but
> should work equally well (I would guess).

[snip]

Well, it is one of the ingredients of Denhardt's solution, after all.

Nick


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