Protein free blocking agent

Nick Theodorakis nicholas_theodorakis at
Fri Oct 29 09:00:02 EST 1999

In article <38195DF5.8A326B2 at>, Fred <dynamin at>
> We use PVP-40 (very cheap from Sigma) to block NC or PVDF for
> western
> blots.  Works extremely well.  Never tried it for hybridisations,
> but
> should work equally well (I would guess).


Well, it is one of the ingredients of Denhardt's solution, after all.


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