How to see small fragment of DNA ?
Chris H Lindley
chris at scotgate2.demon.co.uk
Sat Oct 30 09:04:05 EST 1999
On Fri, 29 Oct 1999 10:38:11 +0200, alaska1 at box43.gnet.pl wrote:
>I`m looking for a system which make a possible checking cutting
>activity of restrictase BspLU11L. I cut some fragmentrs of DNA, but I
>don`t know how to check efficiency of my enzyme. Fragments after
>cutting are 500 and 4 bp big. Is it possible detect that small (4 bp)
Well never done it, but when I order my primers they usually
come with a digitised gel image (PAGE). (no idea of the %
or other stuff) So I would assume if you have enough of the DNA
present a 4bp fragment would show up if my 20bp primers do!!
Perhaps somebody with actual practical experience would comment?
¦ Chris H. Lindley Yorkshire, UK ¦
¦ chris at scotgate2.demon.co.uk Ferg on #os/2 and #os2uk, EFnet ¦
¦ WarpUK:UK OS/2 Users group www.warp.in-uk.net ¦
¦ Molecular Biology & OS/2 www.scotgate.demon.co.uk ¦
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