Freezing down intact organelles

Trond Olav Berg t.o.berg at labmed.uio.no
Thu Sep 2 04:04:18 EST 1999


I am working on an in vitro fusion assay, trying to get isolated,
purified autophagosomes to fuse with lysosomes. It is a lot of work to
isolate these organelles, and it would of course be a large advantage to
freeze them down.

I am therefore looking for good procedures for the freezing down of
intact organelles, of course as gently as possible, so the organelles
are ready to fuse after they are thawed.

Good protocols are appreciated!

Trond Olav Berg




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