BacMam / stability of baculoviral stocks
rkuhelj at worldnet.att.net
Thu Sep 2 16:58:16 EST 1999
High-titer stock of Novagen's BacMam-B-gal baculovirus was generated that
had an initial titer of about 3x10(8) pfu/mL. I was able to successfully
infect HepG2 cell line with these viruses and got about 50 % of the cells
turn blue. However, when I used the same stock after 4 months, there were no
blue cells at all. I haven't had the titer rechecked but i guess it must
have dropped significantly. Does anybody have any experience with BacMam
technology and the long-term stability of BacMam stocks? What is the
stability of baculoviral stocks in general? I kept my stock at 4oC and had
it wrapped in Alu-foil... Is presence of serum crucial for stability? I know
that serum-free media are very popular...
I'd appreciate any help/comment.
rkuhelj at att.net
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