blocking a dna adapter / linker against 5->3 fill in ?

juamber at my-deja.com juamber at my-deja.com
Fri Sep 3 07:49:04 EST 1999


In article <7qjidt$1b0g$1 at gwdu67.gwdg.de>,
  "Ralf Sigmund" <ralf.sigmund at mpihan.mpg.de> wrote:

> Now I would like to block the 3'-terminal base of the bottom linker,
so that Taq-Polymerase cannot fill in the 5'-3' direction.
> I thought about ordering a oligonucleotide with a 2',3'-didesoxy group
in this position.

Why not ordering a modified oligo with an amine group in the 3'
position? It also blocks the taq (Siebert et al. 1995. NAR 23:1087-1088)
Don't know about prices.

Juamber


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