blocking a dna adapter / linker against 5->3 fill in ?

Nicolas von Ahsen nvahsen at hotmail.com
Fri Sep 3 12:14:00 EST 1999


On Fri, 03 Sep 1999 12:49:04 GMT, juamber at my-deja.com wrote:

>In article <7qjidt$1b0g$1 at gwdu67.gwdg.de>,
>  "Ralf Sigmund" <ralf.sigmund at mpihan.mpg.de> wrote:
>
>> Now I would like to block the 3'-terminal base of the bottom linker,
>so that Taq-Polymerase cannot fill in the 5'-3' direction.
>> I thought about ordering a oligonucleotide with a 2',3'-didesoxy group
>in this position.
>
>Why not ordering a modified oligo with an amine group in the 3'
>position? It also blocks the taq (Siebert et al. 1995. NAR 23:1087-1088)
>Don't know about prices.
>
>Juamber
>
>
>Sent via Deja.com http://www.deja.com/
>Share what you know. Learn what you don't.

Or also you may use a 3' phophate group which is easily intruduced by
starting DNA synthesis from a phosphate CPG. Four 0.2µM columns cost
$60 (GlenRes). Almost every Oligo-supplier should be able to do that.
Best regards

Nicolas




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