using UV absorbance scans to 'measure' homogeneity of proteins

Ellis Golub ellis at drakesdrum.dental.upenn.edu
Sun Sep 5 15:57:42 EST 1999


In the old days, one paid particular attention to the 260/280 ratio, as an
indication of the extent of contamination by nucleic acids.  In the original 5
volume set of Methods in Enzymology, there is a table of 260/280 ratios for
mixtures of protein and NA.

Ellis Golub

David wrote:

> Hi,
>
> I've heard that one can tell from the UV absorbance scan of a protein
> solution, whether it is likely to be homogeneous or not. I'd be grateful
> for any comments on this method, e.g. what features of the scan one should
> look for, and how reliable this method is.
>
> Thank you very much in advance,
>
> David




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