Blunt/Sticky-end ligation

Stefan Kappeler stefan.kappeler at ilw.agrl.ethz.ch
Tue Sep 7 09:36:49 EST 1999


I should ligate a 1kb insert into a dephosphorylated 6.5 kb vector. One
end is sticky (XbaI) the other blunt (PmlI). What are the best reaction
conditions:
-temperature, time
-buffer composition (ATP, PEG,...)
-molar ratio insert/vector

Many thanks
Stefan




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