cloning cylinders

Richard P. Grant r_grant at see.sig.for.address
Tue Sep 7 10:00:19 EST 1999

In article <37d50c15.0 at>, "Tobias Straub"
<tobias.straub at> wrote:

>i'm facing serious problems trying to isolate stable mammalian cell clones
>using self-made cloning cylinders.  leaking is the big problem. are there
>any methods that are more reliable?

Limiting dilution?  Works a treat.

You are sealing the cylinders with (e.g.) Vaseline, aren't you?


Richard P. Grant MA DPhil     |             rgrant at
work:        |      home:           
                 -- "I am a doughnut!" --

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