cloning cylinders

Richard P. Grant r_grant at see.sig.for.address
Tue Sep 7 10:00:19 EST 1999


In article <37d50c15.0 at uni-wuerzburg.de>, "Tobias Straub"
<tobias.straub at mail.uni-wuerzburg.de> wrote:

>i'm facing serious problems trying to isolate stable mammalian cell clones
>using self-made cloning cylinders.  leaking is the big problem. are there
>any methods that are more reliable?
>

Limiting dilution?  Works a treat.

You are sealing the cylinders with (e.g.) Vaseline, aren't you?

R

-- 
Richard P. Grant MA DPhil     |             rgrant at cmtech.co.uk
work: www.cmtech.co.uk        |      home: www.avnet.co.uk/adastra           
                 -- "I am a doughnut!" --



More information about the Methods mailing list