cloning cylinders

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Tue Sep 7 17:56:43 EST 1999


In article <37d50c15.0 at uni-wuerzburg.de>, "Tobias Straub" <tobias.straub at mail.uni-wuerzburg.de> wrote:
:i'm facing serious problems trying to isolate stable mammalian cell clones
:using self-made cloning cylinders.  leaking is the big problem. are there
:any methods that are more reliable?
:

Yes. Label clones with the black marker under the microscope, 
get the dish in the hood and pick clone directly with yellow tip
(gentle scapping action helps). Works quite well and allows to get 
great number of clones from the same dish. 

Cross-contamination theoretically might be a problem but is not crucial
in most cases. In those when it has to be eliminated, a second round of 
cloning is required anyway with any method. 

        - Dima




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