get rid of Alkaline phosphatase

KIM dkim at hector.NMSU.Edu
Wed Sep 8 13:00:14 EST 1999


The thing to do is buy Shrimp Alkaline Phosphatase from USB.  It is
heat-inactivated at 65 C for 15 minutes.  Works like a charm.

Daniel Kim
dkim at nmsu.edu

Sue Jin <sjin at stanford.edu> wrote:
: Hi, there

: I am wondering  if  there is a easy way to get rid of the posphatase
: without doing phenol extraction after dephosphorylation of the plasmid DNA
: for subcloning? Your help is appreciated!!

: sue



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