get rid of Alkaline phosphatase
dkim at hector.NMSU.Edu
Wed Sep 8 13:00:14 EST 1999
The thing to do is buy Shrimp Alkaline Phosphatase from USB. It is
heat-inactivated at 65 C for 15 minutes. Works like a charm.
dkim at nmsu.edu
Sue Jin <sjin at stanford.edu> wrote:
: Hi, there
: I am wondering if there is a easy way to get rid of the posphatase
: without doing phenol extraction after dephosphorylation of the plasmid DNA
: for subcloning? Your help is appreciated!!
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